1. The mechanical contraction of circle muscularis strips, the contractile response of dispersed smooth muscle cells, the change of 〓 stimulated by sennoside with different concentration and that stimulated by Ach after incubated with sennoside were evaluated. The translocation of RhoA by sennoside were measured by laser confocal scanting microscopy too.
检测不同浓度的番泻甙对小肠环行肌条和游离环行平滑肌细胞的收缩作用及对〓的影响，然后测定番泻甙对Ach刺激的小肠环行肌条、游离环行平滑肌细胞收缩反应和细胞内〓变化的影响作用，并通过免疫荧光实验检测番泻甙对RhoA的活化和空间转位的影响。